Rajaji Pavadai, Arunjegan Amalraj, Sivanesan Subramanian, Panneerselvam Perumal.Capillary Sensors Composed of CdTe Quantum Dots for Real-Time In Situ Detection of Cu2+. Jin Wang, XueQiong Su, DongWen Gao, RuiXiang Chen, YunYun Mu, XinPing Zhang, Li Wang.This article is cited by 564 publications. The DNAzyme catalytic beacon method demonstrated here can be applied to designing turn-on fluorescent sensors for other paramagnetic metal ions. The sensor selectivity is more than 2000-fold for Cu 2+ over Fe 2+ and UO 2 2+ and more than 10 000-fold over any other metal ions. The sensor has a detection limit of 35 nM and a dynamic range up to 20 μM. Addition of Cu 2+ induced oxidative cleavage of the substrate, and the fluorescence intensity increased by 13-fold. Initially, the fluorescence was quenched. The substrate strand of the DNAzyme was labeled with a fluorophore on the 3‘-end and a quencher on the 5‘-end, and the enzyme strand was labeled with a second quencher on the 5‘-end. We herein report a highly sensitive and selective “turn-on” fluorescent Cu 2+ sensor based on an in vitro selected DNAzyme (also known as catalytic DNA or deoxyribozyme).

Although significant progresses have been made in designing fluorescent sensors for diamagnetic metal ions, few effective Cu 2+ sensors are known because of the paramagnetic nature of the metal ion. Copper is a key metal ion both in environment monitoring and in biology, and exposure to high concentration of copper can cause adverse health effects.

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